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The DETECTER Perl script is available for free download from the Foundation for Applied Molecular Evolution. This script sorts data on inferred ancestral character states supplied by Ziheng Yang's PAML 'rst' output file. This information is then used to predict whether amino acid replacements are tolerated in a modern protein based on the evolutionary analysis of the protein family.

Application of DETECTER, an Evolutionary Genomic Tool to Analyze Genetic Variation, to the Cystic Fibrosis Gene Family
Gaucher, EA; DeKee, DW; Benner, SA
BMC Genomics 7 44 (2006)

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